Classical PNH

About this case study
Details:

Classical PNH

Flow cytometry
Details of flow cytometry instrument

BD FACSCanto

Cell populations evaluated for PNH clones:

Granulocytes, monocytes, RBCs

External control:

None

Internal control:

None

Antibodies/reagents and gating strategies used:

FLAER-Alexa 488, CD14 (61D3)-PE, CD15 (H198)-APC, CD24 (SN3)-PE, CD64 (10.1)-PE, CD45 (2D1)-PerCP-Cy5.5, CD59 (MEM43)-PE, CD235a (KC16)-FITC Practical Guidelines 2012

Clone sizes in all populations tested:

Granulocytes: 98.9% (Type II + III)
Monocytes: 99.8% (Type II + III)
RBCs: 29.7% (Type III), 1.5% (Type II)

LDH level:58.5 µkat/L (2.2–3.6)
Ferritin level:335 µg/L (10–291)
Creatine level:239 µmol/L (44–104)
RBC count:4.01 x 1012/L (4.70–6.10)
WBC and neutrophil counts:4.3 x 109/L (4.00–10.80)
FLAER, fluorescent aerolysin; LDH, lactate dehydrogenase; RBC, red blood cell; WBC, white blood cell

Netflow - Classical PNH

FC data

Netflow - Classical PNH

Dot plots


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