CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

About this case study
Details:	CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone
Flow cytometry
Details of flow cytometry instrument	BD FACSCanto
Cell populations evaluated for PNH clones:	Granulocytes Monocytes
Antibodies/reagents and gating strategies used:	5 colour: FLAER-Alexa 488/CD157-PE/CD45-PerCP-Cy5.5/CD15-APC/CD64-PE-Cy7 6 colour: FLAER-Alexa 488/CD24-PE/CD14-PerCP-Cy5.5/CD15-APC/CD64-PE-Cy7/CD45-APC-H7
Clone sizes in all populations tested:	PNH granulocytes (Type II and III): 5 colour-22.45%, 6 colour-22.62% PNH monocytes (Type II and III): 5 colour-44.75%, 6 colour-44.93%

FC data

Dot plots