CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

About this case study
Details:

CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

Flow cytometry
Details of flow cytometry instrument

BD FACSCanto

Cell populations evaluated for PNH clones:

Granulocytes

Monocytes

Antibodies/reagents and gating strategies used:

5 colour: FLAER-Alexa 488/CD157-PE/CD45-PerCP-Cy5.5/CD15-APC/CD64-PE-Cy7

6 colour: FLAER-Alexa 488/CD24-PE/CD14-PerCP-Cy5.5/CD15-APC/CD64-PE-Cy7/CD45-APC-H7

Clone sizes in all populations tested:

PNH granulocytes (Type II and III): 5 colour-22.45%, 6 colour-22.62%

PNH monocytes (Type II and III): 5 colour-44.75%, 6 colour-44.93%

FLAER, fluorescent aerolysin; WBC, white blood cell

Netflow - CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

FC data

Netflow - CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

Dot plots

Netflow - CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

Dot plots

Netflow - CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

Dot plots

Netflow - CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

Dot plots

Netflow - CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

Dot plots

Netflow - CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

Dot plots

Netflow - CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

Dot plots

Netflow - CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

Dot plots

Netflow - CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

Dot plots

Netflow - CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

Dot plots

Netflow - CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

Dot plots

Netflow - CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

Dot plots

Netflow - CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

Dot plots

Netflow - CD157-based 5-colour vs 6-colour analysis of medium to large PNH WBC clone

Dot plots


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